Muscle protein metabolism in neonatal alloxan-administered rats: effects of continuous and intermittent swimming training.

Carla Ribeiro,Leandro P Moura,Michel B Araújo,Rodrigo A Dalia,Lucieli T Cambri,Gustavo G Araújo,Maria Ar Mello,Ana C Ghezzi,Jose D Botezelli

doi:10.1186/1758-5996-4-5

Carla Ribeiro, Leandro P Moura + Show 7 more

Open Access

https://doi.org/10.1186/1758-5996-4-5

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Abstract

BackgroundThis study aimed to examine the effects of intermittent and continuous swimming training on muscle protein metabolism in neonatal alloxan-administered rats.MethodsWistar rats were used and divided into six groups: sedentary alloxan (SA), sedentary control (SC), continuous trained alloxan (CA), intermittent trained alloxan (IA), continuous trained control (CC) and intermittent trained control (IC). Alloxan (250 mg/kg body weight) was injected into newborn rats at 6 days of age. The continuous training protocol consisted of 12 weeks of swimming training in individual cylinder tanks while supporting a load that was 5% of body weight; uninterrupted swimming for 1 h/day, five days a week. The intermittent training protocol consisted of 12 weeks of swimming training in individual cylinder tanks while supporting a load that was 15% of body weight; 30 s of activity interrupted by 30 s of rest for a total of 20 min/day, five days a week.ResultsAt 28 days, the alloxan animals displayed higher glycemia after glucose overload than the control animals. No differences in insulinemia among the groups were detected. At 120 days, no differences in serum albumin and total protein among the groups were observed. Compared to the other groups, DNA concentrations were higher in the alloxan animals that were subjected to continuous training, whereas the DNA/protein ratio was higher in the alloxan animals that were subjected to intermittent training.ConclusionIt was concluded that continuous and intermittent training sessions were effective in altering muscle growth by hyperplasia and hypertrophy, respectively, in alloxan-administered animals.

Highlights

This study aimed to examine the effects of intermittent and continuous swimming training on muscle protein metabolism in neonatal alloxan-administered rats
Diabetes is a group of metabolic disorders characterized by hyperglycemia resulting from defects in insulin secretion or insulin action [1,2,3], which leads to changes in carbohydrate, fat and protein metabolism
The adaptation consisted of the following sequence, which was followed by the initiation of training: 5, 10, and 15 min in shallow water; 5, 10, and 15 min in deep water; 5 min with a bag tied to the thorax; and 5, 10, and 15 min with a bag containing a load of 3% of body weight tied to the thorax

Summary

Introduction

This study aimed to examine the effects of intermittent and continuous swimming training on muscle protein metabolism in neonatal alloxan-administered rats. In the treatment of T2DM, regular physical exercise at different intensities improves glucose tolerance and reduces insulin resistance. The literature lacks direct evidence on the preventive effect of exercise on the development of T2DM; in particular, investigations into the effect of effort intensity and different exercise protocols is lacking because this type of research is more difficult to conduct in humans. In this context, animal models are more suitable for the study of these issues

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