Muscarinic receptors in the central nervous system of the rat. III. Postnatal development of binding of [ 3H]propylbenzilylcholine mustard

Abstract

The postnatal development of muscarinic receptors has been studied in 7 selected areas from the brains of 1–17-day-old rats by counting silver grains in light microscope autoradiographs of the specific (atropine-sensitive) binding of [ 3H]propylbenzilylcholine mustard in cryostat sections. A major part of the adult receptor density is present at 1 day of age, a time when only a small fraction of the adult number of synapses has yet been formed. Of the areas studied the hypoglossal nucleus is the most precocious in muscarinic receptor development, and the dentate gyrus the latest (associated with the late development of the dentate granule cells). The pattern of receptor distribution changes with development. The caudateputamen first develops receptor in patches, beginning at the lateral (ventricular) surface. The pontine nuclei develop receptor in a medial to lateral sequence. The maturation of the adult laminar pattern of the olfactory bulb depends on the alignment of cells (especially the mitral cells). The neocortex initially has uniform labelling throughout its depth, and later the labelling in layer 4 becomes relatively less dense (probably associated with the ingrowth of afferent fibres). The hippocampal formation first develops receptor evenly over the pyramidal cell dendrites; later receptor appears over the newly formed dentate stratum moleculare and becomes much reduced over the hippocampal stratum lucidum and stratum lacunosummoleculare (probably associated with the ingrowth of afferent fibres from the dentate gyrus and entorhinal area). In the cerebellum muscarinic receptor is found only in the lobules which receive the primary vestibular afferents. In the neonate it is present in the granular layer, but this later disappears and is replaced by the adult pattern of labelling in the molecular layer.