Abstract
Muscarinic receptor types and their effects on the inositol phosphate second messenger system were studied in enzymatically dispersed rat gastric mucosal cells. Radioreeeptor binding studies indicated the presence of a single class of binding sites (4860 ± 875 sites cell ) and affinities of 0.42 ± 0.12 nM, 176 ± 32 nM and 13 μM for N-methylscopolamine, pirenzepine and carbachol, respectively. In cells prelabelled with myo-[ 3H]inositol carbachol induced a dose-dependent increase in inositol-1-phosphate in the presence of lithium with an ED 50 of 10 μM which was antagonized by atropine and pirenzepine (IC 50 9 and 700 nM, respectively). Carbachol stimulated amino[ 14C]pyrine uptake, used as a measure of acid secretion, with an ED 50 of 10 μM. The good correlation between these responses suggests a role for inositol phosphates in the muscarinic M2-receptor mediated acid secretion.
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