Muscarinic cholinergic receptor subtypes in the pigeon bursa of Fabricius: a radioligand binding and autoradiographic study

Abstract

The pharmacological profile and the anatomical localisation of muscarinic cholinergic receptor subtypes were studied in the pigeon bursa of Fabricius, using radioligand binding and autoradiographic techniques with [ 3H]quinuclidinyl benzilate (QNB) as a ligand. [ 3H]QNB was specifically bound to sections of bursa of Fabricius. The binding was time-, temperature- and concentration-dependent. The dissociation constant was 0.31 ± 0.02 nM, and the maximum density of binding sites averaged 38 ± 2.5 fmol/mg protein. The pharmacological profile of [ 3H]QNB binding to sections of pigeon bursa of Fabricius was consistent with the labelling of M 2, M 3 and M 4 muscarinic receptor subtypes. Light microscope autoradiography showed the localisation of [ 3H]QNB binding sites in the medulla, in follicular septa, in the cortico-medullary border and in lesser amounts in the cortical layer. The functional significance of these receptors should be clarified in future studies.