Muricauda hymeniacidonis sp. nov., isolated from sponge of Hymeniacidon sinapium.

Abstract

A Gram-stain-negative, rod-shaped, non-motile, aerobic, orange-pigmented, bacterium (designated 176CP4-71T) was isolated from a sponge sample of Hymeniacidonsinapium from Cheongpo beach, Taean district, in the Republic of Korea. On the basis of 16S rRNA gene sequencing, strain 176CP4-71T clustered with species of the genus Muricauda and appeared to be closely related to Muricaudapacifica LMG 26637T (95.4 %), Muricaudaantarctica JCM 18450T (94.7 %), Muricaudataeanensis KACC 16195T (94.7 %) and Muricaudalutaonensis KCTC 22339T (94.2 %). The average nucleotide identity values between strain 176CP4-71T and the closely related strains were 70.7-73.7 %, indicating that 176CP4-71T is a novel species of the genus Muricauda. Growth occurs at 10-35 °C on marine agar medium in the presence of 1-8 % NaCl (w/v) and at pH 6.0-8.5. The DNA G+C content of the genomic DNA was 41.6 mol %, and menaquinone with six isoprene units (MK-6) was the major respiratory quinone. The major cellular fatty acids (>5 %) were C15 : 0iso, C15 : 1iso G, C17 : 0iso 3OH and C16:1ω6c and/or C16:1ω7c (summed feature 3). The polar lipids consisted of phosphatidylethanolamine, three unidentified aminophospholipids, an unidentified phospholipid and unidentified lipids. Physiological and biochemical characteristics indicated that strain 176CP4-71T represents a novel species of the genus Muricauda, for which the name Muricauda hymeniacidonis sp. nov. is proposed. The type strain is 176CP4-71T (=KACC 19889T=LMG 31033T).