Abstract
Accurate and in vivo characterization of structural, functional, and molecular characteristics of biological tissue will facilitate quantitative diagnosis, therapeutic guidance, and outcome assessment in many clinical applications, such as wound healing, cancer surgery, and organ transplantation. We introduced and tested a multiview hyperspectral imaging technique for noninvasive topographic imaging of cutaneous wound oxygenation. The technique integrated a multiview module and a hyperspectral module in a single portable unit. Four plane mirrors were cohered to form a multiview reflective mirror set with a rectangular cross section. The mirror set was placed between a hyperspectral camera and the target biological tissue. For a single image acquisition task, a hyperspectral data cube with five views was obtained. The five-view hyperspectral image consisted of a main objective image and four reflective images. Three-dimensional (3-D) topography of the scene was achieved by correlating the matching pixels between the objective image and the reflective images. 3-D mapping of tissue oxygenation was achieved using a hyperspectral oxygenation algorithm. The multiview hyperspectral imaging technique was validated in a wound model, a tissue-simulating blood phantom, and in vivo biological tissue. The experimental results demonstrated the technical feasibility of using multiview hyperspectral imaging for 3-D topography of tissue functional properties.
Highlights
Accurate and in vivo characterization of structural, functional, and molecular characteristics of biological tissue will facilitate quantitative diagnosis, therapeutic guidance, and outcome assessment in many clinical applications, such as wound healing, cancer surgery, and organ transplantation
A multiview reflective mirror set was placed in front of the lens of the imager, and the topography of the tissue with an oxygenation map could be obtained with a 3-D reconstruction algorithm and a wide gap second-derivative spectroscopic algorithm
The method was calibrated with a stereo camera calibration method
Summary
Accurate and in vivo characterization of structural, functional, and molecular characteristics of biological tissue will facilitate quantitative diagnosis, therapeutic guidance, and outcome assessment in many clinical applications, such as wound healing, cancer surgery, and organ transplantation. Structural characteristics are measured with a standard phantom or a transparent paper, which are invasive and inconvenient.[1] Optical techniques, such as optical coherence tomography (OCT) and multiview imaging, provide noninvasive, rapid, and quantitative methods for characterizing tissue topography. OCT is able to obtain depth-resolved structural information of biological tissue with an imaging depth up to 3 mm.[2] Multiview images utilize multiple viewpoints and captured multiple pictures of objective tissue to reconstruct three-dimensional (3-D) tissue topography. These methods reveal only 3-D structural information of biological tissue without functional characteristics
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