Multivariate analysis for yield and yield-related traits of amaranth genotypes from Ethiopia

Abstract

The genus Amaranthus is one of the few dicotyledonous, non-grass mesophytes that use specialized C4 annuals or short-lived perennials to produce significant amounts of edible small-seeded pseudo cereals. In this study, we characterized the genetic diversity of 120 genotypes of amaranths collected from diverse amaranth-growing regions of Ethiopia using multivariate analysis of yield and yield-related traits. The experiments were carried out at Hawassa University, in the years 2020 and 2021. The experimental design was set up using an alpha lattice design and replicated two times. The collected data were examined for 24 descriptors. Principal component analysis showed that the first six principal components with eigenvalues greater than one contributed 80.41% of the variability. However, the first two principal components explained 52.42% of the total variation. The highest contributing traits in the first component were days to flowering, basal stem diameter, plant height at flowering, plant height at maturity, auxiliary inflorescence length, number of branches, terminal inflorescence lateral length, days to maturity, terminal inflorescence stalk length, leaf number, leaf length, top lateral branch length. The traits with the greatest weight on the second component were leaf area, basal lateral branch length, leaf length, and leaf width, grain filling period, grain sinking filling rate, and grain yield. Therefore, selection based on these traits would be effective for yield improvement in amaranth genotypes. Additionally, the hierarchical clustering grouped all the genotypes into five clusters. The pairwise generalized squared distance (D2) among the five clusters based on Mahalanobis's D2 statistics revealed the maximum and highly significant genetic distance was observed between II and III (277.79), while the minimum inter-cluster distance observed between clusters I and II (39.50). The findings suggest that amaranth genotypes in Ethiopia have a lot of genetic variation, which might be used for future breeding and ought to be conserved.