Abstract
Objective Lung carcinomas are associated with molecular abnormalities. We performed a pilot study to determine the feasibility of an interphase fluorescence in-situ hybridization (FISH) assay for the detection of lung carcinoma in bronchial washing specimens and tissue biopsy. Materials and methods Twenty bronchial washing specimens were analyzed. The LAVysion multicolor probe set, which contains locus-specific probes to 5p15, 7p12 (EGFR), and 8q24 (CMYC) and a centromeric probe to chromosome 6, was used; hTERT gene (5p15) red and control region EGR1 gene (5q31) green probes were also used. Results FISH positivity in the samples of this study was 60% (12/20) for LAVysion probes and only 33.3% (6/20) for hTERT probe. Conclusion The current study has indicated that multitarget FISH assays have shown higher sensitivity in detecting abnormal cells in bronchial washing and biopsied cells from patients with lung cancer; in addition, hTERT gene amplification or high copy number could be a marker for very poor prognosis in early-stage non-small-cell lung cancer patients.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
