Multistep microsatellite mutation leading to father-child mismatch of FGA locus in a case of non-exclusion parentage.

Abstract

A non-exclusion paternity case with a mismatch in the autosomal short tandem repeats (STR) locus FGA is reported. The genotypes of the suspected father, the mother and the questioned child in FGA locus were 18/25, 20/26 and 20/22, respectively. Examination of 38 autosomal STR loci revealed no mismatches, and the paternity index is up to 1.3618×10(6). The haplotype of 16 Y chromosomal STR in the child matched completely with that of the father. These results suggested that the suspected father is the biological father of the child and that a rare three- or four-step microsatellite mutation had occurred in the paternal allele of FGA.