Abstract
Streptomycetes are known as the producers of various bioactive substances widely used in agriculture and many branches of industry. Different biotechnological methods and approaches are used to increased productivity of streptomycetes. In the present study, S. albus strains were subjected to mutagenesis associated with multistage selection and mutant strain with an increase of 3.5 times bacteriolytic enzyme complex production was obtained. Analysis of cell survival rate, antimicrobial and lytic activity as well as enzyme complex production in Streptomyces exposed to the various mutagen combinations (MNU, UV-irradiation, HNO2) were carried out. Mutant strains were characterized by high bacteriolytic enzyme activity and significant antibiotic activity against Kocuria (Micrococcus) variants and Candida albicans. To estimate genomic changes caused by different mutagens, PCR with primers to short nucleotide repeats was performed and amplicons distinguishing parental and mutant strains were defined. The efficacy of the sequential mutagenic treatment for the hyper-producer obtaining as well as the relationship between the mutagen nature and the genomic variability has been shown. The results obtained in our research can be used and applied to the others Streptomyces species.
Highlights
The traditional methods of microbial producers selection, such as mutagenesis and phenotypic selection, are still of current importance
Streptomycete strains, chosen for multistage selection were obtained by MNU (2435/М strain) and sequential treatment with MNU and UVirradiation (US 101 strain) (Pager, 2010)
Nitrite salt that generates nitric acid at low pH in water solution was used at the stage of mutagenesis
Summary
The traditional methods of microbial producers selection, such as mutagenesis and phenotypic selection, are still of current importance. They are applied both to choose bacterial hyper-producers and as the way of high biosynthetic level maintenance. The last one is the most popular method which has been used for a long period of time to maintain the industrial microbial producers. The current studies, in most cases, include multistage selection after sequential treatment of bacterial culture with several factors. The most useful combination of affecting factors is the chemical and physical mutagens (Rasouli and Kulkarni, 1994; Matseliukh, 1998; Cheng et al, 2001; Liu et al, 2003). The Received 11 May 2014; Revised 24 January 2015; Accepted 01 February 2015; Published Online 01 March 2015
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