Abstract
We have recently developed a novel dual window scheme for processing spectroscopic OCT images to provide spatially resolved true color imaging of chromophores in scattering samples. Here we apply this method to measure the extinction spectra of plasmonic nanoparticles at various concentrations for potential in vivo applications. We experimentally demonstrate sub-nanomolar sensitivity in the measurement of nanoparticle concentrations, and show that colorimetric imaging with multiple species of nanoparticles produces enhanced contrast for spectroscopic OCT in both tissue phantom and cell studies.
Highlights
Spectroscopic optical coherence tomography (SOCT) is a technique that combines the highresolution, noninvasive, three-dimensional imaging capability of optical coherence tomography (OCT) with the ability to obtain spectral information about a sample
In order to enhance the diagnostic ability of SOCT, there has been great interest in the development of contrast agents that can be used to label biological tissues to increase optical contrast compared with traditional OCT methods
The wide bandwidth in METRiCS OCT enables high depth resolution, greater than that seen with most OCT systems operating in the infrared region of the spectrum
Summary
Spectroscopic optical coherence tomography (SOCT) is a technique that combines the highresolution, noninvasive, three-dimensional imaging capability of optical coherence tomography (OCT) with the ability to obtain spectral information about a sample. These previous efforts in using nanoparticles as contrast agents for SOCT have utilized infrared light sources, and have not exploited the ability to obtain simultaneous contrast from endogenous absorbers such as hemoglobin, which absorbs primarily in the visible region of the spectrum and is a compelling target for biomedical applications [4,5] An alternative to these techniques is molecular imaging true-color spectroscopic (METRiCS) OCT which uses a wide spectral bandwidth laser source centered in the visible spectrum and the dual window (DW) processing method [5], which reveals spatially resolved spectroscopic information with high resolution in both the spatial domain and the spectral domain. We demonstrate the enhanced true color contrast provided by this method in combination with nanoparticles of varying types in tissue phantoms and cells
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