Multirresistência e fatores de virulência de Staphylococcus aureus isolados de suínos

M.L.M Nobre,B.C Sousa,L.S Santos,F.A.A Oliveira,M.A.S Campos,A.V Figueirêdo,D.R.P Silva,M.C.S Muratori,M.J.S Soares,A.R Araújo

doi:10.1590/1678-4162-11953

Abstract

ABSTRACT The emergence of livestock-associated methicillin-resistant Staphylococcus aureus strains (LA-MRSA) and the potential role of pigs in the evolution of these strains has led to increased interest in research of these microorganisms. However, this has contributed to a lack of research in the isolation and characterization of methicillin-susceptible S. aureus strains (MSSA). In this study, the prevalence of S. aureus in pigs in the nursery and finishing stages were analyzed. The susceptibility profiles to antibiotics, tolerance to heavy metals, and biofilm production of the isolates were evaluated using phenotypic and genotypic techniques. A total of 1,250 colonies suggestive of Staphylococcus spp. were isolated from 128 pigs, of which 63.6% (n = 795) belonged to this microbial genus. Sixty-seven colonies isolated from 34 animals (26.5%) were confirmed as S. aureus (8.4%). No strains resistant to copper, zinc, or methicillin were detected; however, all strains presented a resistance profile to at least three different classes of antimicrobials and 21 produced biofilms. These data are of concern, as they indicate the need for increased surveillance in the use of antimicrobials as well as reinforce the importance of studies on MSSA strains.

Highlights

The Staphylococcus genus comprises more than 50 species described as commensal microorganisms of mammals and birds, and/or opportunists that cause various clinical manifestations (Schleifer and Bell, 2015)
Such adaptability is reflected in the number of different infectious clinical conditions that S. aureus can cause in humans and animals; these include simple infections, such as those located in the skin, and severe and disseminated diseases, such as bacteremia, endocarditis, pneumonia, meningitis, osteomyelitis, mastitis, arthritis, and toxemia, including food-borne staphylococcal intoxication (Schmidt et al, 2015)
Antimicrobial susceptibility tests were performed using the disc diffusion method according to the M2 document of the Clinical and Laboratory Standards Institute (CLSI, 2018a)

Summary

A.V. Figueirêdo

Multiresistance and virulence factors of Staphylococcus aureus isolated from pigs https://orcid.org/0000-0001-7099-4520 M.C.S. Muratori https://orcid.org/0000-0002-4569-0995. M.J.S. Soares [Multirresistência e fatores de virulência de Staphylococcus aureus isolados de suínos].

INTRODUCTION

MATERIAL AND METHODS

RESULTS AND DISCUSSION

CONCLUSIONS