Abstract
A new analytical method was developed and validated for simultaneous analysis of 423 pesticides, isomers, and pesticide metabolites in nutraceutical products obtained from green tea (Camellia sinensis) extract. Response surface methodology was employed to optimize a generic extraction method. The automated extraction procedure was achieved in a simple disposable pipet extraction. Comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry was used for the separation and detection of all the analytes. The method was validated by taking into consideration the guidelines specified in European SANCO/12571/2013 Guideline 2013 and Commission Decision 2002/657/EC. The extraction recoveries were in a range of 81.6–113.0%, with coefficient of variation <6.4%. The limits of decision for the analytes are in the range 0.04–4.15μgkg−1. The detection capabilities for the analytes are in the range 0.07–6.92μgkg−1. The 423 compounds behave dynamic in the range 0.1–200μgkg−1 concentration, with correlation coefficient >0.99. This validated method has been successfully applied on screening of pesticide residues in one hundred and twenty-four different commercial nutraceutical products from green tea extract, and methamidophos, resmethrin, propoxur, tridemorph, ethiofencarb, flamprop isopropyl, furalaxyl, bifenthrin and fenpropathrin were detected in a few samples tested in this study.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
