Multi-residue Determination of Bisphenol Compounds in Feed Using Ultrasound-Assisted Extraction and Dispersive Solid-Phase Extract Followed by High-Performance Liquid Chromatography with Fluorescence Detector

Abstract

Monitoring of bisphenols (BPs) in feed is a necessary process for the security of food of animal origin. In this study, a method was established to simultaneously detect bisphenol A (BPA), bisphenol B (BPB), bisphenol C (BPC), bisphenol E (BPE), bisphenol F (BPF), bisphenol AP (BPAP), bisphenol Z (BPZ), bisphenol P (BPP) and bisphenol PH (BPPH) in feed. A simple and rapid pretreatment procedure was developed using ultrasound-assisted extraction (UAE) and dispersive solid-phase extraction (DSPE), and then the prepared sample was analysed by high-performance liquid chromatographic (HPLC) with fluorescence detector (FLD). Finally, the operating conditions of UAE, DSPE, and HPLC were all optimised to increase the sample throughput and sensitivity. Under the optimized conditions, good linearities were obtained for nine BPs with the correlation coefficients (R2) above 0.995; the recoveries of nine BPs in spiked feed samples were in the range of 82.56–103.87% and relative standard deviations (RSDs) were in the range of 2.4–13.40%; the limits of detections (LODs) and quantitations (LOQs) were in the range of 1.0–5.0 and 3.3–16.6 μg kg − 1, respectively. The results clearly demonstrated the developed method provided the reliable screening and quantification of nine BPs in feed and possessed the advantages including low cost, quick, and simple.