Abstract
We have developed an automated, highly sensitive system for the detection of chemiluminescent reactions that are rapid or burst-type reactions. Approximately 25 samples per hour can be run with human myeloperoxidase-H 2O 2-high Cl −. This is due to the difficulties of washing out cationic myeloperoxidase from the flow cell. Various nonsteroidal anti-inflammatory drugs were run against the enzyme and the most inhibitory were ascorbate and BW755C at doses around 10 −7 M, while sulindac sulfide and indomethacin were the most inhibitory at 10 −6 M range. Aspirin and salicylate were partially effective at the 10 −5 M range. This type of CL system should be generally useful for a variety of fast CL reactions, because it is automated, with continuous recording, and integration of peaks is possible.
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