Abstract
Selective, multipurpose electrodes have been developed from the previously described glucose electrode based on amperometric detection of hydrogen peroxide. Several single or multi-enzyme systems, including galactose oxidase, cholesterol oxidase, glucoamylase with glucose oxidase, and invertase with glucose oxidase, can be covalently bound to collagen membranes and attached to a platinum anode for monitoring the hydrogen peroxide generated. The probes allow fast and sensitive measurements of galactose, free cholesterol and maltose. Analogous electrodes are convenient for the assay of sucrose and lactose, with lower sensitivity. For disaccharide measurements, a comparative study of membranes produced by random co-immobilization, stacking of membranes and asymmetric coupling is reported. Asymmetric coupling improved the electrode performances in every case. One enzyme membrane is readily replaced by another in the electrode construction, and the sensors can be used for hundreds of assays.
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