Abstract
The caudal neural plate is a distinct region of the embryo that gives rise to major progenitor lineages of the developing central and peripheral nervous system, including neural crest and floor plate cells. We show that dual inhibition of the glycogen synthase kinase 3β and activin/nodal pathways by small molecules differentiate human pluripotent stem cells (hPSCs) directly into a preneuroepithelial progenitor population we named “caudal neural progenitors” (CNPs). CNPs coexpress caudal neural plate and mesoderm markers, and, share high similarities to embryonic caudal neural plate cells in their lineage differentiation potential. Exposure of CNPs to BMP2/4, sonic hedgehog, or FGF2 signaling efficiently directs their fate to neural crest/roof plate cells, floor plate cells, and caudally specified neuroepithelial cells, respectively. Neural crest derived from CNPs differentiated to neural crest derivatives and demonstrated extensive migratory properties in vivo. Importantly, we also determined the key extrinsic factors specifying CNPs from human embryonic stem cell include FGF8, canonical WNT, and IGF1. Our studies are the first to identify a multipotent neural progenitor derived from hPSCs, that is the precursor for major neural lineages of the embryonic caudal neural tube. Stem Cells 2015;33:1759–1770
Highlights
In the embryo, specification of early neural progenitors begins soon after gastrulation with the formation of the neural plate, which subsequently folds to become the embryonic neural tube [1, 2]
We show that dual inhibition of the glycogen synthase kinase 3b and activin/nodal pathways by small molecules differentiate human pluripotent stem cells directly into a preneuroepithelial progenitor population we named “caudal neural progenitors” (CNPs)
Our previous studies described a novel OCT42/SOX21/ PAX62 progenitor derived from human pluripotent stem cells (hPSCs), which is induced by dual inhibition of the glycogen synthase kinase 3b (GSK3b) and Activin/Nodal pathways mediated by CHIR and SB treatments, respectively [10]
Summary
Specification of early neural progenitors begins soon after gastrulation with the formation of the neural plate, which subsequently folds to become the embryonic neural tube [1, 2]. The caudal neural plate gives rise to the caudal diencephalon, mesencephalon, and metencephalon and the spinal cord. Recent studies have demonstrated that the caudal neural plate region arises from bipotent mesoectodermal progenitors, known as axial stem cells, which coexpress mesodermal and neural markers including Sox and Brachyury [4, 5]. Axial stem cells can generate either neural or mesodermal lineages, their fate being regulated by persistent expression of Sox or onset of Tbx expression, respectively [4, 6]
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.