Abstract

There have been only two brief reports which presented direct evidence of the existence of more than one monoamine oxidase in mitochondria although this has been suggested by many workers. Gorkin (1963) partially separated p-nitrophenylethylamine oxidase activity from m-nitro-p-hydroxybenzylamine oxidase activity by ion exchange chromatography of mitochondrial extracts in the presence of nonionic detergent (OP-10). Youdim and Sandler (1967) report the appearance of two or three bands of enzyme activity upon polyacrylamide gel electrophoresis of partially purified monoamine oxidase from rat liver or human placental mitochondria. This paper describes the separation of solubilized liver mitochondrial monoamine oxidases from three species by gel filtration in the presence of detergent. Based on substrate specificity there appear to be at least three different monoamine oxidases present in rat and rabbit liver and at least two in beef liver.

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