Abstract
A simple method for the in vitro cultivation of chick embryo skin epithelial cells was developed and the replication of turkey herpes virus (HVT) and Marek's disease herpes virus (MDHV) in this system was studied. A high percentage of cells in monolayers inoculated with HVT (19·7 per cent) and MDHV (4·6 per cent) was infected on the fourth day post-incubation. Titres of infectious cells in the culture fluid or cell-free virus in the cell extract were low and cell-free virus was not detected in the culture medium. Enveloped particles of HVT, but not of MDHV, were detected by electron microscopy.
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