Abstract
To evaluate the growth rate of M. lepraemurium on the course of cultivation in cellfree liquid medium, a new estimation method for this purpose was established, and named the Sediment Smear Method (SSM). The procedure is as follows: centrifugalize culture tubes at 2, 500 rpm for 30 min, discard carefully the culture fluid by a capillary pipet, resuspended collected sediment in 0.1 ml of a formaline-gelatin solution, smear this suspension by a loop on a slide glass, and stain, then observe the bacterial mass under the low magnification. The results obtained illustrate there is roughly a parallel relationship with the increase of the number of bacilli measured by a bacillary counting method. Therefore, the SSM could be recommended as a simple screening method for course evaluation of the growth of M. lepraemurium, may be as well as M. leprae, in the cellfree cultivation experiments.
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