Abstract
Cells from the established line NHIK 3025 were exposed to haematoporphyrin derivative and light. After this photodynamic treatment the first interphase of surviving cells was prolonged. Furthermore, a pronounced effect on the progression through the first mitosis was observed. Mainly the duration of metaphase was increased. Some of the cells were irreversibly arrested in mitosis and the cells that were able to complete mitosis after treatment multiplied in the subsequent generations at the same rate as the control. Cells treated in the late stages of the mitosis went out of mitosis at the same rate as the control. This indicates that the treatment with porphyrins and light induces a block in a specific stage of mitosis.
Highlights
Summary.-Cells from the established line NHIK 3025 were exposed to haematoporphyrin derivative and light
The dose-dependent growth-delay demonstrated is mainly due to inhibition in the first cell cycle after treatment with porphyrins and light
Mitosis is the stage which is mainly influenced by the treatment, a small prolongation of interphase has been observed (Fig. 2)
Summary
A rapid lysis and loss of colony-forming ability of NHIK 3025 cells has been seen in vitro after treatment of cells with haematoporphyrin (HP) and light The cell population selected contained > 9000 mitosis (see Fig. 4) as reported previously (Pettersen et al, 1977). To cells has been shown to be repaired to Chemicals.-HPD was made from haematoporphyrin di-hydrochloride (Koch-Light) after the procedure of Lipson et al (1961) and stored at - 18°C in the dark. The mitotic index was calculated from differential counting of living cells in the inverted phase-contrast microscope. As control experiments, staining of the chromosomes with aceto-orcein or haematoxylin was done with replicate cultures and the mitotic index was calculated from differential counting of stained cells.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
