Abstract
The goal of this project was to evaluate the embryogenic callus induction of two Coffea arabica clones selected for their characteristics of rust resistance and high yield, as well as to compare their multiplication in two different media under both solid and liquid cultivation conditions. The protocol described by Teixeira et al. (2004) was used for callus induction in a randomized block design in which each clone was considered a treatment. Evaluation of callus induction was carried out 180 days after initiation by counting embryogenic calli. For callus multiplication, the treatments consisted of two different media (stage two of Albarran et al. (2004) and the multiplication medium described by Teixeira et al. (2004)) and two cultivation systems (solid and liquid). Evaluations were conducted by weighing calli 21, 42 and 63 days after initiation of the experiment. The two studied clones exhibited the same potential for embryogenic callus induction. The potential for embryogenic callus multiplication was influenced by the plant's genotype. When compared with the liquid system, the solid system displayed the highest level of embryogenic callus multiplication for the clones studied.
Highlights
Somatic embryogenesis has been achieved in a great number of families and species of plants and has been used for basic studies of vegetable physiology and in more practical applications, such as micropropagation and genetic transformation
There was no significant difference between the clones studied (X1) in the chi-squared test, demonstrating that their genotypes had no effect on the formation of embryogenic calli (Table 1)
And MichauxFerriere (1996) obtained embryogenic callus formation starting at 20.8% from various genotypes of C. canephora when a primary medium was used containing 2.2 μM 2,4-D, 4.9 μM IBA and 9.8 μM 2-iP
Summary
Somatic embryogenesis has been achieved in a great number of families and species of plants and has been used for basic studies of vegetable physiology and in more practical applications, such as micropropagation and genetic transformation. The potentially important application of embryogenesis for the accelerated multiplication of coffee plants was quickly noticed by the earliest studies, and numerous techniques that simplify this process have been developed that seek to establish. Embryogenic calli can continue to originate somatic embryos for long time periods and after many subcultures. According to Pasqual et al (1997), continuous somatic embryo production depends on the continuous proliferation of proembryogenic nodules from young somatic embryos during each subculture. Some authors have described protocols seeking to optimize the multiplication of embryogenic coffee calli
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
