Abstract
Organ formation has been studied with lateral buds excised from bulbs of Iris hollandica cv.‘Wedgwood.’The buds were cultured in vitro on the medium of modified Kano, Neergarrd and Morel. Adventitious bud formation of explants was induced on the medium containing both NAA and BA, but root formation was suppressed. Root formation was observed on the medium containing NAA alone as a plant growth regulator. High concentration of mineral salt favoured the growth of explants, and better results were obtained on the medium based on Morel or modified Kano. Peptone added into modified Kano′s medium inhibited the growth of explants at 2g/l and higher concentrations. The growth of explants was good at pH 5.0 and 6.0. The optimal concentrations for agar and sucrose were 2g/l and 2-4%, respectively.
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