Abstract
Nymphs of aster leafhoppers were allowed to feed for 2 days on diseased plants of China aster to acquire aster yellows virus. No virus was recovered when juices of these leafhoppers were injected into virus-free leafhoppers on the following day. However, virus was recovered by the same injection method after the nymphs had been cut into pieces and their living tissues incubated for 10 days in a suitable medium in hanging drops. This experiment demonstrated that aster yellows virus could complete its incubation not only in vectors feeding on a constant supply of fresh plant food, but also in insect tissues in vitro. The experiment provided additional evidence for multiplication of this plant virus in the tissues of its insect vector.
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