Multiplication of a myxovirus (SV5) with minimal cytopathic effects and without interference

Abstract

SV5 virus, a simian myxovirus, multiplies to high titer in rhesus monkey kidney cells, but causes only minimal cytopathic effects. It forms plaques with high efficiency, the plaques consisting of intact cells with diminished neutral red staining.After inoculation of SV5 virus at a multiplicity of 40–70 PFU/cell, the latent period was 6–7 hours, and the doubling time during the period of exponential increase in infective virus was approximately 50 minutes. In 24 hours, yields of 500–1500 PFU/cell were obtained. Cells continued to produce high yields of infective virus for periods up to 30 days.Inoculation of SV5 virus at high multiplicity did not result in autointerference or incomplete virus formation. Multiplication of SV5 virus in monkey kidney cells for 48 hours did not interfere with subsequent infection and plaque formation by a second virus, and did not significantly reduce the yield of a superinfecting virus.