Abstract
Coherent control can be used to selectively enhance or cancel concurrent multiphoton processes, and has been suggested as a means to achieve nonlinear microscopy of multiple signals. Here we report multiplexed two-photon imaging in vivo with fast pixel rates and micrometer resolution. We control broadband laser pulses with a shaping scheme combining diffraction on an optically-addressed spatial light modulator and a scanning mirror allowing to switch between programmable shapes at kiloHertz rates. Using coherent control of the two-photon excited fluorescence, it was possible to perform selective microscopy of GFP and endogenous fluorescence in developing Drosophila embryos. This study establishes that broadband pulse shaping is a viable means for achieving multiplexed nonlinear imaging of biological tissues.
Highlights
Coherent control is attracting considerable interest as a way to enhance or cancel specific photoinduced multiphoton processes through quantum interference [1, 2, 3, 4]
We control broadband laser pulses with a shaping scheme combining diffraction on an optically-addressed spatial light modulator and a scanning mirror allowing to switch between programmable shapes at kiloHertz rates
This study establishes that broadband pulse shaping is a viable means for achieving multiplexed nonlinear imaging of biological tissues
Summary
Coherent control is attracting considerable interest as a way to enhance or cancel specific photoinduced multiphoton processes through quantum interference [1, 2, 3, 4].
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