Multiplexed protein MRM assay panel for high throughput toxicological assessment in rat liver samples

Abstract

Introduction Early prediction of drug toxicity remains challenging in pre-clinical toxicological studies. Especially on the protein level there is a lack of methods enabling discovery and validation of predictive biomarker signatures in animal or cell-based models exposed to toxic insults. Multiple reaction monitoring (MRM), a massspectrometric approach, enables monitoring of up to 100 proteins in a single measurement with high specificity and accuracy if optimal assays are available.