Multiplexed optofluidic laser immunosensor for sensitive and rapid detection of biomarkers

Abstract

Multiplexed biomarker detection is highly desirable for the early diagnosis and treatment of diseases. Optofluidic laser has shown extraordinary ability in single biomarker detection, but the simultaneous detection of multiple biomarkers remains unexplored. Here, we develop optofluidic laser immunosensors based on multidimensional division multiplexing technologies. Time division multiplexing (TDM) based optofluidic lasers are demonstrated by the temporal control of the immunoreaction and sequential generation of immunocomplexes. Spatial/wavelength division multiplexing (SDM/WDM) based optofluidic lasers are achieved by integrating microfluidic channels and exploiting different emission bands. The feasibility of the multiplexed laser immunosensors is demonstrated by the detection of dual-biomarkers of acute myocardial infarction and lung cancer. The multiplexed laser immunosensor features the superimposed enhancement effects of laser amplification and latex particles, enabling a limit of detection down to the fM (10−15 molar) level. The assay time can be shortened to 15 min thanks to the utilization of homogeneous immunoreaction. Based on the multiplexed detection of biomarkers, our technology takes a significant step toward the early diagnosis of major diseases.