Abstract
AbstractThis paper reports a dual immunosensor for the simultaneous determination of two important fertility‐related hormones: 17β‐estradiol, E2, and follicle‐stimulating hormone, FSH. The implemented method involves direct competitive (E2) or sandwich‐type (FSH) immunoassays carried out on magnetic microparticles (MBs) and amperometric detection at screen‐printed dual carbon electrodes (SPdCEs) involving the hydroquinone (HQ)/H2O2 system. The developed immune platform demonstrates LOD values of 6.88 pg mL−1 and 0.11 mIU mL−1 for E2 and FSH standards, respectively and usefulness for the determination in saliva samples collected from different volunteers, giving results in agreement with the conventional ELISA methodologies.
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