Multiplex PCR via Microarray a Surrogate for Rapid Molecular Detection of Mycobacterial and Fungal Species Along with Anti-Microbial Resistant Genes, Directly from Clinical Specimens’

Abstract

Multi drug resistant Mycobacterial and invasive fungal infections imposes immense Global health burden resulting in high morbidity and mortality rates. The biggest delima is the time consuming culture and sensitivity diagnostics i.e 04 to 06 weeks for Mycobacterial and 02 to 04 weeks for fungal infections. The delay in result provision adds up to patient’s miseries especially for resistant cases. Therefore the aim of writing this short commentary was to introduce multiplex PCR via microarray bead hybridization assay. A thorough literature review was found extremely deficient. However, a limited published data concluded this technique as a surrogate for rapid molecular and resistant gene depiction for mycobacterial and fungal species directly from clinical specimens. Another biggest advantage was simultaneous genomics and resistant genes identification for Mycobacterial and Fungal isolates. The resistant genes for 1st line anti tuberculous therapy (ATT), 2nd line ATT and commonly used anti fungals can be detected easily. This cost effective test can be finalized in approximately less than 24 hours. Thus early and accurate management can be timely started for both said infections.