Multiplex PCR using newly designed primers for very short fragments of TH01, TPOX, CSF1PO, and vWA loci

Abstract

We performed multiplex PCR for the TH01, TPOX, CSF1PO, and vWA loci using newly designed pairs of primers that yield smaller fragments than previously reported [Int. J. Leg. Med. 114 (2001) 285; Am. J. Hum. Genet. 55 (1994) 175; Int. J. Leg. Med. 106 (1994) 183.] [ 1–3]. This system required genomic DNA in a range of 50 pg–2 ng, and proved to be sensitive as a typing method. Furthermore, it was possible to determine the allele types even from 18-year-old bloodstains.