Abstract
Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. Defining SEOV genome sequences plays a critical role in development of preventive and therapeutic strategies against the unique worldwide hantavirus. We applied multiplex PCR–based next-generation sequencing to obtain SEOV genome sequences from clinical and reservoir host specimens. Epidemiologic surveillance of R. norvegicus rats in South Korea during 2000–2016 demonstrated that the serologic prevalence of enzootic SEOV infections was not significant on the basis of sex, weight (age), and season. Viral loads of SEOV in rats showed wide dissemination in tissues and dynamic circulation among populations. Phylogenetic analyses showed the global diversity of SEOV and possible genomic configuration of genetic exchanges.
Highlights
Seoul virus (SEOV) poses a worldwide public health threat
Retrospective Analysis of hemorrhagic fever with renal syndrome (HFRS) Patient Specimens We found that specimens collected in 2002 from 6 HFRS patients were positive for SEOV by ELISA
We confirmed that the HFRS specimens were serologically positive for SEOV by immunofluorescence antibody (IFA) (Table 1)
Summary
Seoul virus (SEOV) poses a worldwide public health threat. This virus, which is harbored by Rattus norvegicus and R. rattus rats, is the causative agent of hemorrhagic fever with renal syndrome (HFRS) in humans, which has been reported in Asia, Europe, the Americas, and Africa. To enrich the low amount of viral RNA, we developed a multiplex PCR–based NGS that showed high coverage of HTNV genome sequences from HFRS patients [19]. We collected 1,269 R. norvegicus rats in an urban HFRS-endemic area in South Korea during 2000–2016. Viral load for SEOV showed ranges from tissues of 5 rats (Rn02-15, Rn10134, Rn10-145, Rn11-44, and Rn11-53) that were positive by serologic and molecular screening (IFA+ PCR+).
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