Abstract
BackgroundIt is essential to detect carriers of carbapenemase-producing Enterobacterales in order to implement infection control measures. The objectives of this study was to evaluate the NG-Test® CARBA 5 (CARBA 5) assay for detection of five carbapenemases and to assess the cross reactivity of other OXA-type carbapenemases with the OXA-48-like specific antibodies.MethodsA total of 197 Enterobacterales isolates were tested. To evaluate the cross reactivity, 73 carbapenem-resistant A. baumannii, harboring OXA-type variants, were tested. Polymerase chain reaction (PCR) served as gold standard for carbapenemase identification.ResultsExcellent agreement was found between PCR and CARBA 5, for all but one isolate. The single false positive result (a blaSME positive S. marcescens isolate) was incorrectly positive for blaOXA-48 by CARBA 5. No cross reactivity was observed. The sensitivity and specificity were 100.0% and 98.0%, respectively.ConclusionsThe CARBA 5 assay is highly sensitive and specific and is recommended as a tool for the detection of the main carbapenemases of interest in clinical microbiology laboratories.
Highlights
Enterobacterales are a common cause of both community-acquired and hospital-acquired infections [1, 2]
If CARBA 5 detected the presence of a carbapenemase, but it differed from the carbapenemase detected by Polymerase chain reaction (PCR), we considered the result a false positive (FP)
Confirmation of the initial screening for carbapenemase identification results was performed by PCR
Summary
Enterobacterales are a common cause of both community-acquired and hospital-acquired infections [1, 2]. These bacteria can acquire genes encoding multiple antibiotic resistance mechanisms, including extendedspectrum β-lactamases (ESBLs), AmpCs, and carbapenemases [3]. CARBA 5 enables the detection of the five most prevalent carbapenemase enzymes (KPC, OXA-48-like, VIM, IMP, and NDM) through the use of specific antibodies [5]. It is essential to detect carriers of carbapenemase-producing Enterobacterales in order to implement infection control measures. The objectives of this study was to evaluate the NG-Test® CARBA 5 (CARBA 5) assay for detection of five carbapenemases and to assess the cross reactivity of other OXA-type carbapenemases with the OXA48-like specific antibodies
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