Abstract
Abstract The determination of serum immunoglobulin isotype concentrations is an important indicator of immunological health. We have constructed several multiplex isotyping assays on the Luminex® assay platform. These assays allow for the analysis of human, mouse and rat serum, tissue culture or body fluid samples. The human isotyping assay enables the quantification of immunoglobulins A, M, G1, G2, G3 and G4 in a single assay well. While the mouse isotyping assay cover immunoglobulins A, M, G1, G2A, G2B, and G3, and the rat isotyping immunoglobulins A, E, M, G1, G2A, G2B, G2C. Since the concentrations of IgE in human and mouse samples are typically much lower than the other immunoglobulin isotypes we have also designed separate assays for mouse and human IgE analysis. This collection of assays allows for the rapid, multiplex analysis of major immunoglobulin isotypes in the human , as well as the two predominant research maodels, rat and mouse.
Published Version
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