Abstract
Drink or food is easy to be contaminated by multiple foodborne pathogens simultaneously. Demand for developing multiplex bioassay platforms is urgent and increasing. Here, we report a quantitative assay for the simultaneous detection of manifold foodborne pathogens by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), magnetic separation and metal nanoclusters-mediated signal amplification. In the first preconcentration step, the sample was mixed with magnetic nanoprobes and metal nanoclusters probes to form sandwich-type magnetic nanoprobes-targets-nanoclusters probes structures. The target bacteria were isolated and concentrated and then unbound metal nanoclusters probes were collected by magnetic separation. In the second step, free metal nanoclusters probes as element tags were etched in 5% HNO3 for ICP-MS analysis and multiplex bacteria detection. The method was able to detect Staphylococcus aureus, Vibrio parahaemolyticus and Salmonella typhimurium simultaneously and successfully detected three targets as low as 10 CFU/mL in ∼30 min. Applications to the determination of target bacteria in food samples confirmed the feasibility of this multiplex assay.
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