Abstract
The alkaloids present in Adhatoda vasica Nees. namely vasicine and vasicinone are responsible for its pharmacological property. Due to exploitation of the plant for various medicinal uses, there is a need to conserve this plant. High frequency and maximum number of multiple shoots were obtained from shoot tip explants harvested during the month of May on MS medium supplemented with BAP (22.20 μm). Rest of the year the explants were turning brown or black after a day of inoculation because of phenolic substances present in the explant. High frequency of rooting was recorded on MS basal medium. The rooted plantlets were hardened and established at 50-60%.
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