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Abstract
1. 1. A new method for separation of particles, such as cells, is described. It is based on sedimentation at 1 g. The separation obtained by a single sedimentation is increased by a multistage procedure analogous to counter-current distribution between two immiscible phases. 2. 2. The principle of the method is described. 3. 3. Applications of the method on starch grains, Sephadex-gel particles, polystyrene latex particles and chlorella cells are described. In all cases separation according to size is obtained. The apparatus used is a modification of a thin-layer counter-current distribution apparatus described earlier (Anal biochem 11 (1965) no. 1; Science tools 17 (1970) no. 3). 4. 4. The method shows a comparatively high selectivity and it is a mild method towards cells since no foreign material has to be included in the suspension medium.
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