Abstract
In this study, effects of different solvents (ethanol, methanol and water) on bioactive performance of defatted grape seed powder (GSP) were investigated using simplex lattice mixture design approach. Also, multiple response optimization process was applied to determine the best solvent type for the high bioactive GSP extract production. For this purpose, the bioactive compound concentrations and their antioxidant and antiradical properties were characterized and the effect of solvent type on the processing variables was modelled. Total phenolic and flavonoid contents of GSP ranged between 0.31-7.29 mg GAE/g and 44.3-537.4 mg CE/kg sample. In addition to that, DPPH and ABTS.+ radical scavenging activity of the samples were in the range of 2.11-80.5% and 0.31-4.08 μg Trolox/ g sample. The effect of solvent type showed a significant effect on all studied bioactive parameters and the best solvent mixture was determined as ethanol (33.84%), methanol (20.17%) and water (45.99%) by the considering the all studied parameters.
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