Abstract
Virgin female boll weevils, Anthonomus grandis Boheman, from a mutant strain with pearl-colored eyes were mated in various combinations to males of the same pearl strain or to males with brown (wild-type) eyes to study the use of sperm in multiple matings. Sperm from the last mating took precedence over sperm from other matings only when at least 24 hours separated the matings. The advantage of sperm from the last mating ranged from about 10% when 1 day separated matings to 90% when 4 days intervened. This advantage lasted from about 7 days when the interval between matings was short to more than 45 days from the longest interval. It is concluded from other data that most field females will follow the pattern of sperm use shown by closely spaced multiple matings.
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