Abstract
Brown planthopper (BPH), one of the most important pests of the rice (Oryza sativa) crop, becomes catastrophic under severe infestations and causes up to 60% yield loss. The highly disastrous BPH biotype in the Indian sub-continent is Biotype 4, which also known as the South Asian Biotype. Though many resistance genes were mapped until now, the utility of the resistance genes in the breeding programs is limited due to the breakdown of resistance and emergence of new biotypes. Hence, to identify the resistance genes for this economically important pest, we have used a multi-parent advanced generation intercross (MAGIC) panel consisting of 391 lines developed from eight indica founder parents. The panel was phenotyped at the controlled conditions for two consecutive years. A set of 27,041 cured polymorphic single nucleotide polymorphism (SNPs) and across-year phenotypic data were used for the identification of marker–trait associations. Genome-wide association analysis was performed to find out consistent associations by employing four single and two multi-locus models. Sixty-one SNPs were consistently detected by all six models. A set of 190 significant marker-associations identified by fixed and random model circulating probability unification (FarmCPU) were considered for searching resistance candidate genes. The highest number of annotated genes were found in chromosome 6 followed by 5 and 1. Ninety-two annotated genes identified across chromosomes of which 13 genes are associated BPH resistance including NB-ARC (nucleotide binding in APAF-1, R gene products, and CED-4) domain-containing protein, NHL repeat-containing protein, LRR containing protein, and WRKY70. The significant SNPs and resistant lines identified from our study could be used for an accelerated breeding program to develop new BPH resistant cultivars.
Highlights
Brown planthopper (BPH) (Nilaparvata lugens (Stål)), an outbreak-prone insect pest, causes heavy yield loss in rice (Oryza sativa) production ecologies
A number of quantitative nucleotides (QTNs) against BPH in rice have been reported in this study
We considered the p value
Summary
Brown planthopper (BPH) (Nilaparvata lugens (Stål)), an outbreak-prone insect pest, causes heavy yield loss in rice (Oryza sativa) production ecologies. It has become a serious economic threat to rice production in several parts of Asia including India due to the rampant use of nitrogenous fertilizers and prophylactic insecticide applications. The famine death of one million people due to a severe attack of BPH was reported in Japan during 1732 [1]. The first and most severe outbreak of BPH with considerable economic damage in India was observed during 1973–1974, which had resulted in damage of almost 50,000 ha of rice crop [2,3]. The mode of damage is a combination of two ways: 1. by laying egg masses in the midrib of the leaf sheath and blade blocking the xylem and phloem, and 2
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
