Multiple forms of immunoreactive dynorphin in human pituitary and pheochromocytoma

Abstract

Multiple forms of immunoreactive dynorphin (I-Dy) in human pituitary and pheochromocytoma were examined utilizing gel filtration and high performance liquid chromatography (HPLC). Gel filtration of I-Dy from these tissues revealed the major component in the position of Dy(1–17) and other minor components with large molecular weight forms. HPLC profile of this major component from gel filtration showed a large peak corresponding to the position of Dy(1–17) and small peaks corresponding to the positions of Dy (1–13), (1–12) and other unknown peptides. These results strongly suggest the presence of Dy(1–17) as the major component, and Dy (1–13), (1–12) or other unknown peptides as the minor components in these human tissues.