Abstract
Bacillus sp. KK01, which was isolated from soil, produced β-mannansse about 2 U ml −1 in culture medium. By DEAE-ion exchange column chromatography, four enzyme components F1, F2, F3, and F4 were separated. All components showed maximum activity at pH 7.1. F1 showed maximum activity at 60°C, which was 5–10°C higher than those of the rest. The four components F1, F2, F3, and F4 hydrolyzed copra mannan to the extent of 23–31%; yielded mannobiose, mannotriose, and mannotetraose as hydrolysis products; and also hydrolyzed locust bean gum to the extent of 19–22%, giving similar products.
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