Abstract
The quality of polysaccharides from different regions was studied by using multiple fingerprint analysis and chemometric analysis. Polysaccharides from 10 batches of Glehnia littoralis were compared based on Fourier-transform infrared spectroscopy (FT-IR), high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), and proton nuclear magnetic resonance (1H-NMR). According to the results, the 10 batches of polysaccharides from G littoralis had high similarity by analyzing HPLC, FT-IR, 1H-NMR, and GPC fingerprints. Through cluster analysis, samples and adulterants in different regions could be classified. Three monosaccharides (galactose, glucose, and galacturonic acid), molecular weights (4.33 × 105-4.91 × 105, 4.04 × 104-4.71 × 104, and 5.02 × 103-5.83 × 103), and H-1 (4.99, 5.39, and 5.42 ppm) of α-glucose could be used as markers for quality control of medicinal materials of the roots of G littoralis.
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