Abstract
Multiple enzyme studies in the vaginal fluid in relation to benign and malignant gynaecological conditions.
Highlights
In general it may be said that women with a gynaecological lesion tend to have a higher secretion of vaginal fluid, in this series there will tend to be a greater number of cases with elevated levels unassociated with carcinoma
Benign gynaecological lesions.-One can have a moderate amount of lactic dehydrogenase in the absence of 6-phosphogluconate dehydrogenase in women with benign gynaecological lesions
In cases with gynaecological lesions, aldolase gave a lower incidence of elevated levels than 6-phosphogluconate dehydrogenase
Summary
Samples were obtained in a manner similar to that of Bonham and Gibbs (1962); 6-phosphogluconate dehydrogenase was assayed by the methods of Glock and McLean (1953). Of vaginal debris present in the reaction mixture, the activity per gram is given by the following formula units per gram = x x 10y00. A modification of the ultraviolet method of Bruns (1954) was used In this assay the enzyme activity is determined by linking the reaction to the ultraviolet by the use of triose phosphate isomerase and glycerophosphate dehydrogenase. Bruns unit is defined as that amount of enzyme activity which removed the 0-0446 ,umoles of fructosel-6 diphosphate per hour at 250 C. In this assay, 0-892 ,tmoles of nicotinadenine dinucleotide are reduced during the conversion of 0.0446 ,umoles of fructose 1-6 diphosphate to triose phosphate. The difference between the activity from cell one and two gives a measure of the activity due to glucose-6-phosphate dehydrogenase
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