Multiple effects of ZnO nanoparticles on goldfish (Carassius auratus): Skin mucus, gut microbiota and stable isotope composition

Abstract

The skin and the gut are direct target tissues for nanoparticles, yet attention to effects of metal-based nanoparticles (MNPs) on these two and the discrepancy in these effects remain inadequate. Here, effects of ZnO nanoparticles (nZnO) on skin mucus and gut microbiota of goldfish (Carassius auratus) were investigated, as well as further elements turnover and metabolic variations. After 14 days of exposure, considerable variations in levels of biomarkers (protein, glucose, lysozyme and immunoglobulin M) in skin mucus demonstrated significant stress responses to nZnO. nZnO exposure significantly reduced the abundance of Cetobacterium in the gut while increased that of multiple pathogens, and further leading to down-regulation of pathways such as carbohydrate metabolism, translation, and replication and repair. Decreased δ15N values indicated declined N turnover in vivo, further demonstrating the negative effect of nZnO on metabolism in the organism. Integration analysis of each biomarker using the biomarker response index version 2 (IBRv2) revealed concentration-dependent effects of nZnO on skin mucus, while effects on physiology in vivo was not, demonstrating the discrepancy in the toxicity pathways and toxic effects of nZnO on different tissues. This work improved our understanding about the comprehensive toxicity of nZnO on aquatic organism.