Multiple effects of mutualistic ants improve the performance of a neotropical ant-plant: A long-term study with the Cecropia-Azteca system

Abstract

Comprehension of the benefits involved in mutualisms is crucial to disentangle the role of interactions in the structure and functioning of populations, communities and ecosystems. In ant-plant mutualisms, benefits provided by plants to ants are immediately recognizable, but reverse benefits are less obvious, conditional and accumulate over longer time spans. Here we tested the hypothesis that the ant Azteca muelleri simultaneously provides multiple benefits to its host plant (Cecropia glaziovii), ultimately increasing plant performance. We planted seedlings and experimentally prevented ant colonization for half of them. Over 4.5 years we quantified the effects of ant presence or absence on plant growth, herbivory levels, fungal infection, fertilization via ant debris and changes in defense strategies. Ant colonization increased plant height by 125% compared to ant-free plants. Such an improvement in plant performance can be explained because plants with ants faced less herbivory, lower prevalence of pathogenic fungi, invested less in foliar trichomes and had more foliar nitrogen. We thus confirmed that ant mutualists provide cumulative benefits including nutritional benefits, effective defense and lower investment into other defenses – which result in increased plant growth. We highlight the importance of long-term experiments that simultaneously evaluate a multiplicity of potential ant effects to better understand their relative contribution to the performance of the mutualistic partner.