Multiple Effects of Dithiothreitol on Nonphotochemical Fluorescence Quenching in Intact Chloroplasts (Influence on Violaxanthin De-epoxidase and Ascorbate Peroxidase Activity).

Abstract

Reversible nonphotochemical fluorescence quenching depends on thylakoid lumen acidification and violaxanthin de-epoxidation and is correlated with photoprotection of photosynthesis. The O2-dependent electron flow in the coupled Mehler-ascorbate peroxidase reaction (MP-reaction) mediates the electron flow necessary for lumen acidification and violaxanthin de-epoxidation in isolated, intact chloroplasts. Inhibition of violaxanthin de-epoxidation by dithiothreitol (DTT) was correlated with suppression of fluorescence quenching. In addition, DTT was also found to suppress fluorescence quenching due to inhibition of ascorbate peroxidase activity, a main enzyme of the MP-reaction, even in the presence of zeaxanthin. In intact, non-CO2-fixing chloroplasts, violaxanthin and antheraxanthin de-epoxidation and the ascorbate peroxidase activity show different sensitivities to increasing DTT concentrations. Violaxanthin de-epoxidase activity, measured as the sum of zeaxanthin and antheraxanthin formed, was inhibited with an inhibitor concentration for 50% inhibition (I50) of 0.35 mM DTT. In contrast, inhibition of the O2-dependent electron flow and corresponding lumen acidification occurred with higher I50 values of 2.5 and 3 mM DTT, respectively, and was attributed to inhibition of ascorbate peroxidase activity (I50 = 2 mM DTT). Accordingly, the DTT-induced inhibition of the nigericin-sensitive nonphotochemical fluorescence quenching was correlated linearly with the decreasing concentrations of zeaxanthin and antheraxanthin and was almost unaffected by DTT inhibition of the MP-reaction and correlated [delta]pH. The nigericin-insensitive, photoinhibitory kind of nonphotochemical fluorescence quenching up to 1 mM was mainly correlated with inhibition of violaxanthin de-epoxidation. At higher DTT concentrations, it was attributed to inhibition of both violaxanthin de-epoxidation and MP-reaction. The results show that DTT has multiple, but distinguishable, effects on nonphotochemical fluorescence quenching in isolated chloroplasts, necessitating careful interpretation.