Abstract
Husavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs' nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study.
Highlights
The number of novel and divergent viruses in the order Picornavirales was significantly increased by next-generation DNA sequencing (NGS) [1,2,3,4,5]
We found that the nucleotide similarity in the polyprotein region of Brazilian sequences is 92%
We report here the first presence of HuV for the first time in Brazil in five children in the urban and rural areas of North and Northeast states
Summary
The number of novel and divergent viruses in the order Picornavirales was significantly increased by next-generation DNA sequencing (NGS) [1,2,3,4,5]. Caliciviridae, Dicistroviridae, Iflaviridae, Marnaviridae, Picornaviridae, Polycipiviridae, Secoviridae and Solinviviridae [3, 4] are classified into eight recognized families in this order. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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