Abstract
Summary A shoot-derived cDNA library of soybean Glycine max (L.) cv. Bragg inoculated with Bradyrhizobium japonicum was constructed for the purpose of assessing expression changes in autoregulated legume plants. To evaluate the representativeness and utility of the library, cDNA clones homologous to yeast cdc2 / CDC28 gene were isolated. Based on restriction and sequence analysis of their 5′ and 3′ regions, two families of genes were found, suggesting that as many as six different cdc2 homologues exist in soybean. These genes may have functional relevance in tissue-specific regulation of cell division. We conclude that the library has utility for nodulation research, especially aspects dealing with shoot-controlled components of autoregulation.
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