Abstract
The general polymerase chain reaction (PCR) amplifies DNA and analyzes the amplification results of the quantified DNA. Recently, real-time PCR has been developed to detect DNA amplification in various ways. The conventional camera-based system is too expensive and difficult to reduce device size. In this paper, we propose a low-cost, compact fluorescence detection system for real-time PCR systems using an open platform camera. To simplify the optics, four low-cost small cameras were fixedly placed, and the entire tube was divided into four quadrants to minimize the field of view. In addition, an effective image processing method was used to compensate. The proposed system measured the fluorescence detection performance on the basis of the amount of DNA using various fluorescent substances.
Highlights
The polymerase chain reaction is an important technique in biological research because it can check diseases by small amounts of DNA [1]
The DNA detection process consists of DNA extraction, DNA amplification, electrophoresis, and gel image analysis
The method of using a photodiode is typically measured after establishing an optical system using a dichroic mirror
Summary
The polymerase chain reaction is an important technique in biological research because it can check diseases by small amounts of DNA [1]. In order to solve this disadvantage, if the tubes be photographed at one time are divided into several groups, and the images obtained from each can be synthesized into one, the real-time PCR apparatus can be compact and inexpensive. This paper, we propose a low-cost, compact fluorescence detecare divided into severalin groups, and the images obtained from each can be synthesized tion system for a real-time. A case was into one, the real-time PCR apparatus can be compact and inexpensive Inmade this using parts that cana be purchased on the market, and a system self-made paper, we propose low-cost, compact fluorescence detection forintegrated a real-timeboard was used. An effective image method was were used the optical component fluorescence measurement, fourprocessing low-cost small cameras to compensate for the entire reduction thedivided signal-to-noise of thetosuperimposed image. It can be applied to a system for measuring various fluorescence that requires a wide field ofand view
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